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Cell cycle assay of 2D- and 3D-cultured A549 cells. (A) Flow cytometry for cell cycle detection at 24 and 48 h after cultivation in each system. Cell number distribution in each cell phase at 24 h (B) and 48 h (C) in 2D and 3D systems (n = 3). The results showed lower division rate of spheroids on scaffolds than cells grown in 2D culture plates. ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns: P > 0.05.
