TABLE 2.
Results of testing the effects of three DNA extraction methods on DNA yields from P. putida cells, B. globigii endospores, and F. moniliforme conidiaa
| Organism | Probabilityb
|
||
|---|---|---|---|
| 70°C incubation in TEN buffer | Three freeze-thaw cycles | Bead mill homogenization | |
| P. putida | 0.002 | 0.029 | 0.047 |
| B. globigii prepn 93 | NSD | NSD | 0.0001 |
| B. globigii prepn 95 | NSD | NSD | 0.0001 |
| F. moniliforme | NSD | NSD | 0.0002 |
The three DNA extraction methods were described in detail in Materials and Methods. Data were analyzed by using indicator variables in the general linear model as described in the text.
Probability of a greater F value (P values) when testing whether the extraction procedure had an effect on the DNA yields as described by SAS type III sums of squares. P values greater than 0.05 were considered significant. NSD, values that were not significantly different (P values of 0.18 to 0.95).