Comparison of the AlgFPaN- and C-terminal domains.A, superposition of N- (model 9) and C-terminal (model 1) domains with an RMSD of 1.35 Å. The average backbone RMSD for the N- and C-terminal domain ensembles is 2.1 Å. B, structural superimposition of the N- and C-terminal domains of AlgFPa with the AlphaFold model of AlgFPa. C, electrostatic potential surface representations of the N- and C-terminal domains (left and right, respectively). Shown in same orientation as panel A. The coulombic potential range from −10 (red) to 10 (blue) kcal/(mol ⋅ e). D, ConSurf analysis of conserved residues in the N- and C-terminal domains (left and right, respectively). Shown in same orientation as panels A and C. Surface representation colored by level of conservation (green variable to purple highly conserved). E, sequence alignment (as in Fig. 1) colored by conservation level.