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ITC analyses of AlgFPp29-215protein–protein interactions with AlgJPp75-370and AlgXPp. Protein–protein interactions were investigated by binary titrations of AlgJ:AlgF (left), AlgF:AlgX (center), and AlgJ:AlgX (right) using ITC. Data were fit and analyzed with the Origin software package as shown, and Kd values were determined. The AlgJ:AlgX interaction (right) was too weak to be analyzed.isothermal titration calorimetry.
