TABLE 3.
Primers/probes used for RT-qPCR.
| Gene | Assay on demand a |
|---|---|
| ALPL | Hs00758162_m1 |
| ANKH | Hs01064613_m1 |
| ENPP1 | Hs01054040_m1 |
| IBSP | Hs00173720_m1 |
| PHOSPHO1 | Hs01370290_m1 |
| PPARγ | Hs00234592_m1 |
| SP7 | Hs00541729_m1 |
| SPP1 | Hs00959010_m1 |
| SOX9 | Hs00165814_m1 |
| Gene | Forward | Reverse | Probe |
| COL1A1 | 5′-CCC TGG AAA GAA TGG AGA TGA T-3′ |
5′-ACT GAA ACC TCT
GTG TCC CTT CA-3′ |
5′-CGG GCA ATC CTC
GAG CAC CCT-3′ |
| RPLP0 | 5′-TGG GCA AGA ACA CCA TGA TG-3′ | 5′-CGG ATA TGA GGC AGC AGT TTC-3′ | 5′-AGG GCA CCT GGA AAA CAA CCC AGC-3′ |
| RUNX2 | 5′-AGC AAG GTT CAA CGA TCT GAG AT-3′ |
5′-TTT GTG AAG ACG
GTT ATG GTC AA-3′ |
5′-TGA AAC TCT TGC
CTC GTC CAC TCC G-3′ |
ALPL: alkaline phosphatase, biomineralisation associated; IBSP: integrin binding sialoprotein; SP7: Sp7 transcription factor; RPLP0: ribosomal protein lateral stalk subunit P0; SPP1: osteopontin, organic component of bone matrix.
a
TaqMan® Gene Expression Assay (Applied Biosystems®).