Thawing media for cryopreserved human bone marrow/peripheral blood mononuclear cells
| Reagent | Amount |
|---|---|
| Fetal Bovine Serum (FBS) | 5 mL |
| DNase I (10 mg/mL) | 500 μL |
| FACS buffer | 45 mL |
| Total | 50.5 mL |
Store at 4°C for a maximum of two weeks.
Thawing media for cryopreserved human bone marrow/peripheral blood mononuclear cells
| Reagent | Amount |
|---|---|
| Fetal Bovine Serum (FBS) | 5 mL |
| DNase I (10 mg/mL) | 500 μL |
| FACS buffer | 45 mL |
| Total | 50.5 mL |
Store at 4°C for a maximum of two weeks.