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| Reagent | 1 reaction |
|---|---|
| Q5 High-Fidelity 2× Master Mix | 5 μL |
| gDNA template 50–150 ng/μL | 0.5 μL |
| Nuclease-free water | 3.5 μL |
| Forward pre-amplification primer 2 μM | 0.5 μL |
| Reverse pre-amplification primer 2 μM | 0.5 μL |
