| Reagent | 1 reaction | 30 reactions |
|---|---|---|
| Q5 High-Fidelity 2× Master Mix | 8 μL | 240 μL |
| Pre-amplification forward primer (locus 1 sample 1) 6.4 μM | 0.25 μL | 7.5 μL |
| Pre-amplification reverse primer (locus 1 sample 1) 6.4 μM | 0.25 μL | 7.5 μL |
| Pre-amplification forward primer (locus 2 sample 1) 6.4 μM | 0.25 μL | 7.5 μL |
| Pre-amplification reverse primer (locus 2 sample 1) 6.4 μM | 0.25 μL | 7.5 μL |
| Nuclease-free water | 1 μL | 30 μL |