Fig. 1.

Structure ofVitis viniferaUDP-Glucose:flavonoid 3-O-glycosyltransferase (VvGT1) in Michaelis complex with UDP-2-deoxy-2-fluoro glucose (UDP-2FGlc, non-hydrolysable donor) and kaempferol (acceptor) (PDB ID: 2C1Z). The cartoon shows the overall structure of VvGT1, with the ligands displayed in ‘ball-and-stick' representation. The β-sheets in both N-terminal domain (NTD, residues 1–242) and C-terminal domain (CTD, residues 260–456) are shown in yellow, while the α-helices in the NTD and CTD are depicted in blue and green, respectively. Loops and the linker connecting the NTD and CTD are presented in gray and pink, respectively. The ligand atoms are color-coded using the CPK scheme, with oxygen in red, nitrogen in blue, fluorine in green and phosphorus in orange. All α-helices and β-strands are labeled to facilitate subsequent analysis. This figure was generated using UCSF Chimera.