Table 2.
Quantification of splice events between two situations for sensitivity determination
| Amount of isoforms in samples A:B | Isoform | Fold changes (B/A) | Relative abundance (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| RE1 | RE2 | RJ | Sample A (Reference) | Sample B (Experimental) | |||||
| LF + SF | LF | SF | LF (40%) | SF (60%) | LF (80%) | SF (20%) | |||
| 1.25 : 2.5 | Theoretical fold changes | 2 | 4 | 0.66 | Isoform quantity (ng) | 0.5 | 0.75 | 2 | 0.5 |
| NM_006904 | 1.75 | 4.06 | 0.74 | 30.4 | 69.6 | 70.5 | 29.5 | ||
| NM_005243 | 1.89 | 5.43 | 0.45 | 28.9 | 71.1 | 83.1 | 16.9 | ||
| NM_001513 | 1.77 | 3.03 | 0.56 | 48.9 | 51.1 | 83.9 | 16.1 | ||
| 0.08 : 0.16 | Theoretical fold changes | 2 | 4 | 0.66 | Isoform quantity (ng) | 0.03 | 0.05 | 0.12 | 0.03 |
| NM_006904 | 1.61 | 2.57 | 0.71 | 48.3 | 51.7 | 77.2 | 22.8 | ||
| NM_005243 | 1.34 | 2.64 | 0.59 | 36.5 | 63.5 | 72.1 | 27.9 | ||
| NM_001513 | 1.65 | 2.16 | 1.2 | 46.8 | 53.2 | 61.4 | 38.6 | ||
Long forms (LFs) and short forms (SFs) of three different genes have been mixed together at various proportions (40% LF, 60% SF for A; 80% LF, 20% SF for B). In each case, the amount of sample B was twice the amount of sample A. The expected fold changes and relative abundance (%) are reported in bold. Fold changes have been measured and the resulting values were used in our calculation method to determine the relative abundance of the long and short isoforms for each gene.