TABLE 2.
Activities of anaplerotic enzymes, enzymes of the glyoxylic shunt, and enzymes mediating oxidative reactions of the citric acid cycle in extracts of L. lactis subsp. lactis NCDO 2118
| Enzyme | Sp acta |
|---|---|
| Anaplerotic and glyoxylic shunt enzymes | |
| PEP carboxylaseb | 3 |
| Malic enzymec | ND |
| Isocitrate lyaseb | ND |
| Malate synthaseb | ND |
| Citric acid cycle enzymes | |
| Citrate synthaseb | 22 |
| Aconitasec | ND |
| NAD-dependent isocitrate dehydrogenaseb | 23 |
| NADP-dependent isocitrate dehydrogenaseb | 3 |
| α-Ketoglutarate dehydrogenaseb | ND |
a
Specific activity was expressed as nanomoles per minute per milligram of protein. The values were average values obtained during measurements with cell extracts from the four synthetic media used in fermentor cultures. ND, not detected.
b
Activity was assayed in the physiological direction.
c
Activity was assayed in both directions.