TABLE 3.
Activities of enzymes mediating the synthesis of glutamate from α-ketoglutarate in extracts of L. lactis subsp. lactis NCDO 2118
| Enzyme | Sp acta |
|---|---|
| Glutamate dehydrogenasec | ND |
| GOGATb | 4 |
| Amino acid-ketoglutarate transaminase | |
| Nitrogen donor amino acids tested | |
| Isoleucine | 142 |
| Leucine | 137 |
| Valine | 69 |
| Methionine | 32 |
| Aspartate | 22 |
| Alanine | 6 |
| Serine | ND |
a
Specific activity was expressed as nanomoles per minute per milligram of protein. The values were average values obtained during measurements with cell extracts from the four synthetic media used in fermentor cultures. ND, not detected.
b
Activity was assayed in the physiological direction.
c
Activity was assayed in both directions.