REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS-CoV-2 (COVID-19) Spike RBD antibody, (Clone: HL1003-HU)	GeneTex	Cat# GTX635866; RRID:AB_2888584
SARS-CoV-2 (COVID-19) Spike RBD antibody, (Clone: HL1003)	GeneTex	Cat# GTX635792; RRID:AB_2888574
SARS-CoV-2 (COVID-19) Spike antibody, (Clonality: Polyclonal)	GeneTex	Cat# GTX135356; RRID:AB_2887482
Biological samples
Serum or Plasma from SARS-CoV-2 vaccinated or Un-vaccinated	Emory Hospital, Atlanta, GA, USA.	N/A
Chemicals and Recombinant proteins
Dulbecco’s Modified Eagle Medium/Ham F-12 50/50 Mix (DMEM F12)	Corning	Cat#10-090-CV
TrypLE™ Express Enzyme (1X), phenol red	Gibco	Cat#12605010
Pen-strep (5000 IU/mL, 5 mg/mL), 100 mL	MPBiologicals	Cat#1670049
DPBS	Gibco	Cat#14190-144
2-Mercaptoethanol	Gibco	Cat#21985023
Bovine Serum Albumin (BSA)	Fisher Bioreagents	Cat#BP1600-100
Sodium Chloride	Sigma	Cat#S3014
Sodium phosphate monobasic monohydrate	Sigma	Cat#S3522
Imidazole	Sigma	Cat#5513
Mini-PROTEAN® TGX™ Precast Gels	Bio Rad	Cat#4561084
Amicon® Ultra-15 Centrifugal Filter Unit	Millipore	Cat#UFC905008
Amicon®Ultra-4 Centrifugal Filter Unit	Millipore	Cat#UFC805008
Amicon® Ultra-0.5Centrifugal Filter Unit	Millipore	Cat#UFC503024
Amicon® Ultra-15 Centrifugal Filter Unit	Millipore	Cat#UFC903024
NativeMark™ Unstained Protein Standard	Thermo Fisher Scientific	Cat#LC0725
Corning® 500 mL Polycarbonate Erlenmeyer Flask with Vent Cap	Corning	Cat#431145
ExpiFectamine™ 293 Transfection Kit	Thermo Fisher Scientific	Cat#A14524
Expi293™ Expression Medium	Thermo Fisher Scientific	Cat#A1435102
HisPur Ni-NTA Resin	Thermo Fisher Scientific	Cat#88221
Opti-MEM™ I Reduced Serum Medium	Thermo Fisher Scientific	Cat#31985070
Pierce™ Protein G Agarose	Thermo Fisher Scientific	Cat#20399
Stericup filters	Thermo Fisher Scientific	Cat#290-4520
Slide-A-lyzer Dialysis Cassette	Thermo Fisher Scientific	Cat#66030
50x TAE (Tris/Acetic Acid/EDTA) Buffer, 5 L	BioRad	Cat#1610773
Transfection Reagent - BioT (1 mL)	Bioland Scientific	Cat#BioT1
Pierce™ Protein G IgG Binding Buffer	Thermo Fisher Scientific	Cat#21011
Pierce™ IgG Elution Buffer	Thermo Fisher Scientific	Cat#21009
TWEEN® 20	Sigma	Cat#P9416-100 mL
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail	Sigma	Cat#11836170001
Bio-Safe™ Coomassie Stain	BioRad	Cat#1610786EDU
Falcon® 96-well White/Clear Flat Bottom TC-treated Microplate, with Lid, Sterile,	Corning	Cat#353377
White 96-well Microplate with Clear Bottom, Sterile and Tissue Culture Treated, Lid Included	PerkinElmer	Cat#6005181
Pierce™ BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23227
Anti-His Biosensors	ForteBio	Cat#18-5114
ProA Biosensors	Sartorius	Cat#18-5010
EDAC, 1-Ethyl-3-(3-Dimethylaminopropyl)carbodiimide, Hydrochloride	Thermo Fisher Scientific	Cat#E2247
Streptavidin-β-galactosidase (SBG)	Quanterix	Cat# 103798
Resorufin ß-D-galactopyranoside (RGP)	Quanterix	Cat#103159
Homebrew beads-750	Quanterix	Cat#103529
Homebrew beads-488	Quanterix	Cat#103526
Bead Conjugation buffer	Quanterix	Cat#101357
Biotinylation Reaction buffer	Quanterix	Cat#101358
Blocking buffer	Quanterix	Cat#101356
Detector/Sample Diluent buffer	Quanterix	Cat#101359
Bead Diluent	Quanterix	Cat#100458
SARS-CoV-2 lentiviral kit	BEI-Resources	Cat# NR-53816
Bright-Glo kit	Promega	Cat#E2610
SARS-CoV-2 Spike (pre-fusion) trimer-Wuhan-WT	This study	jroback@emory.edu
SARS-CoV-2 Spike (pre-fusion) trimer-Delta	This study	jroback@emory.edu
SARS-CoV-2 Spike (pre-fusion) trimer-Omicron BA1	This study	jroback@emory.edu
SARS-CoV-2 Spike (pre-fusion) trimer-Omicron-BA2	This study	jroback@emory.edu
Experimental models: Cell lines
HEK-293T	ATCC	Cat#CRL-3216
HEK-293T-ACE-2	BEI-Resources	Cat#NR-52511
Expi-293	Thermo Fisher Scientific	Cat#A14527
Software and algorithms
Prism 10	GraphPad	https://www.graphpad.com/
PyMoL	Schrödinger	https://pymol.org/2/