Figure 3.
Fucoxanthin suppressed TBI-induced endothelial cell apoptosis
(A and B) TUNEL staining was used to examine the percentage of TUNEL+ and CD31+ cells at 3 days after TBI and fucoxanthin i.c.v. injection.
(C–E) Western blot assay for the expression of Bax and Bcl-2 in the ipsilateral cortex at 3 days after TBI and fucoxanthin i.c.v. injection.
(F and G) Western blot assay for the expression of cleaved caspase-3 in the ipsilateral cortex at 3 days after TBI and fucoxanthin i.c.v. injection. n = 3 per group.
Data were presented as mean ± SD; ∗∗∗p < 0.001 versus sham group; ###p < 0.001 versus TBI + vehicle group. Scale bar of TUNEL staining: 20 μm β-actin was used as a loading control.