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. 2023 Nov 13;223(1):e202203005. doi: 10.1083/jcb.202203005

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© 2023 Wu et al.

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Figure 3. — USP37 interacts with ERK1/2. (A and B) HEK293T cells were transfected indicated deubiquitinases (DUBs) plasmid. After transfection for 48 h, cells were lysed and purified using anti-HA-agarose beads. Then coimmunoprecipitating endogenous ERK1/2 was detected by anti-ERK1/2 antibody. (C and D) The cancer cell lysates were subject to immunoprecipitation with control IgG, anti-ERK1/2 (C), or anti-USP37 (D) antibodies. The immunoprecipitates were then blotted with the indicated antibodies. (E) HEK293T cell lysates were incubated with GST or GST-USP37 proteins. After washing, proteins bound on sepharose were blotted with indicated antibodies. (F) HEK293T cells were transfected with the indicated constructs. Cells were lysed and cell lysates were purified on anti-HA-agarose, and then coimmunoprecipitating endogenous ERK1/2 was detected by anti-ERK1/2 antibody. Source data are available for this figure: SourceData F3.