Table 1.
Yeast strains used in the study
| Name | Genotype | Reference |
|---|---|---|
| PC313a | MATaura3-52 | (40) |
| PC538a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 | (17) |
| PC673a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 ste20::kanMX6 | (17) |
| PC986b | MATα his3Δ0, leu2Δ0, met15Δ0, ura3Δ0 | (131) |
| PC1894a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 leu2::HYG | (28) |
| PC2382 | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 ste12::kanMX | (28) |
| PC3391a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 rga1::NAT | (28) |
| PC3392a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 ste12::kanMX6 rga1::NAT | (28) |
| PC3393a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 bem4::HYG rga1::NAT | (28) |
| PC3551a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 leu2::HYG bem4::NAT | (28) |
| PC3862a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 ste11::NAT | (28) |
| PC5851b | MATaura3-52 his3-200 ade2-101 his3-200 leu2-1 | (70) |
| PC5852b | MATaura3-52 his3-200 ade2-101 his3-200 leu2-1 cim3-1 | (70) |
| PC5024a | MATaura3-52 ste11::NAT | (28) |
| PC6016a,c | MATacan1Δ::Ste2pr-spHIS5 lyp1Δ::Ste3pr-LEU2 his3::hisG leu2Δ0 ura3Δ0 | (132) |
| PC6102a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 tec1::NAT | (101) |
| PC6539a | MATaste4 FUS1-lacZ FUS1-HIS3 ura3-52 cdc42::NAT pRS316-GFP-linker-CDC42 | (65) |
| PC6591a | MATaura3-5 leu2 | (65) |
| PC6810a,d | MATaura3-52 leu2 ssk1 | (29) |
| PC6604a | MATaura3-52 leu2 ssk1 ste11::NAT | (29) |
| PC6684a | MATaura3-52 leu2 ssk1 cdc42::NAT pRS316-GFP-linker-CDC42 | (29) |
| PC6687a | MATaura3-52 leu2 ssk1 rga1::NAT | (29) |
| PC6936a | MATaura3-52 cdc24::NAT leu2::HYG pRS315 cdc24-4 | (74) |
| PC7501a | MATaura3-52 cdc24::NAT leu2::HYG pRS315 cdc24-4 pRS316 | This study |
| PC7772a | MATaura3-52 leu2 ssk1 ste20::NAT | This study |
| PC7777a | MATaste4 FUS1-lacZ FUS1-HIS3 ste20::NAT rga1::HYG | This study |
| PC7780a | MATaste4 FUS1-lacZ FUS1-HIS3 ste20::NAT bem4::HYG | This study |
| PC7886a | MATaste4 FUS1-lacZ FUS1-HIS3 bem4::HYG rga1::NAT ste20::GENT | This study |
Bold indicates that it is a historical designation for this mating type nomenclature in yeast.
a
Strains are Σ1278b background.
b
S288c background.
c
Strains from an ordered deletion collection described in (132) were also used.
d
Strains containing three CDC42 alleles (K5A, V36T, and F37Y) were constructed in the Σ1278b background and described in (29).