Table 2.
Plasmids used in the study
| Name | Description | Reference |
|---|---|---|
| PC1441 | YCp50-STE11-4 | (133) |
| PC1696 | pMsb2-GFP | (85) |
| PC1715 | pRS316-SHO1(P120L)-GFP | (85) |
| PC2150 | pRS316-CDC24 | (28) |
| PC1422 | pRS315 | (124) |
| PC2769 | pV84 | (134) |
| PC2107 | pCDC24-lacZ | This study |
| PC2207 | pRS316 | (124) |
| PC4394 | pRL116 CEN/URA3 GFP-Ste20 | (35) |
| PC4395 | pRL116 CEN/URA3 GFP-Ste20334-369Δ (CRIBΔ) | (35) |
| PC6455 | pRS306-GFP-linker-CDC42 (pDLB3609) | (34) |
| PC6454 | pRS316-GFP-linker-CDC42 | (29) |
| PC6457 | pRS315-GFP-linker-CDC42 | (29) |
| PC7458 | pRS316-GFP-linker-CDC42 (Q61L) | (65) |
| PC7654 | pRS316-GFP-linker-CDC42 (Q61L+ TD: K5R, Q61L, K94R, K96R) | (65) |
| PC3621 | pSW17 control plasmid | (99) |
| PC3622 | pSW72 CDC24 | (99) |
| PC3623 | pSW76 CDC24 with PH-A and Linker A mutations | (99) |
| PC3624 | pSW78 CDC24 with PH-A mutations | (99) |
| PC3625 | pSW80 CDC24 with Linker A mutations | (99) |
| PC3626 | pSW82 CDC24 with T35A mutations | (99) |
| PC7670a | pRS316-GFP-linker-CDC42 (V36T) | This study |
a
The pBMH1 and pBMH2 plasmids are driven by the GAL1 promoter and have been described in (90).