FIG. 2.

Protein fractionation analysis of streptavidin-conjugated single-chain anti-B. cereus spore T antibody. (A) SDS-12% PAGE; (B) Western blot. Fusion protein was probed with rabbit anti-streptavidin antiserum (Sigma S6390) and anti-rabbit IgG peroxidase conjugate (Sigma A8275). Lanes: MW, molecular weight standards; 1, total cell sample (without IPTG induction); 2, total cell sample (with 0.2 mM IPTG induction); 3, osmotic shock fraction; 4, soluble fraction from cell lysis; 5, final solubilized cellular fraction.