TABLE 1.

Fractions of B. cereus spores^a recovered from the phosphate buffer system by the single-chain antibody-coated immunomagnetic beads

Type of beads	Fusion protein immobilization treatment	Incubation temp (°C)	% of sporesb			Total (%)
			Unbound	Bound but removed by washing	Bound
Biotinylatedc	+	23	1.7 (0.6)	0.3 (0.2)	98.0 (0.8)	100
Biotinylated	+	4	9.3 (3.4)	0.7 (0.2)	90.0 (3.6)	100
Biotinylated	−	4	99.3 (0.5)	0.6 (0.4)	0.1 (0.08)	100
Biotinylated	−	23	98.6 (0.6)	1.3 (0.6)	0.1 (0.6)	100
Originald	−	23	94.3 (3.4)	5.0 (3.0)	0.7 (0.5)	100
Original	+	23	64.4 (7.8)	32.4 (7.1)	3.2 (1.6)	100

^aSpore concentration, 5 × 10⁴ spores/ml; incubation time, 60 min. 

^bAverages from three trials (numbers in parentheses are standard deviations). 

^cCommercial sheep anti-mouse IgG-coated magnetic beads which were biotinylated as described in Materials and Methods. 

^dCommercial sheep anti-mouse IgG-coated magnetic beads.