TABLE 2.

Fractions of B. cereus spores^a recovered from pasteurized whole milk^b by the single-chain-antibody-coated immunomagnetic beads

Type of beads	Incubation (°C)	% of sporesc			Total (%)
		Unbound	Bound but removed by washing	Bound
Immunomagneticd	4	62.3 (11.5)	0.7 (0.3)	37.0 (11.2)	100
Controle	4	99.0 (0.1)	0.9 (0.1)	0.1 (0.05)	100

^aSpore concentration, 5 × 10⁴ spores/ml; incubation time, 60 min. 

^bThe aerobic plate count of milk sample was <2 × 10² CFU/ml before spiking with B. cereus spores. 

^cValues are averages of three trials (numbers in parentheses are standard deviations). 

^dStreptavidin-conjugated single-chain antibodies were immobilized on biotinylated magnetic beads. 

^eThe beads were biotinylated by N-hydroxysuccinimidobiotin, but the streptavidin-conjugated antibody was not added.