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. 2023 Oct 30;68:102949. doi: 10.1016/j.redox.2023.102949

Fig. 7.

Fig. 7

AMPK isomer siRNA reduces hypoxic CSE phosphorylation and per-polysulfide formation. MAEC were treated with si-Con, siAMPKα1 or siAMPKα2 was examined for AMPKα1 and AMPKα2 mRNA expression (Panels A and B). Panel C shows representative blots of pCSES346, total CSE, pAMPK, total AMPK and GAPDH from MAEC treated under hypoxia with siCon, siAMPKα1, or siAMPKα2. Panels D and E respectively show quantitation of pCSES346 and pAMPK protein levels from immunoblots in Panel C. Panel F illustrates SSP4 levels from MAEC transfected with siCon, siAMPKα1, or siAMPKα2 under hypoxia. Densiometric analysis of p-AMPK and p-CSE346 blots were normalized to total AMPK or total CSE (normalized to GAPDH for protein). All data are averaged from triplicates from each experiment with at least n = 5. ****P < 0.0001; ***P < 0.0002; **P < 0.003; *P < 0.01.