Figure 3.
The Pcdh19E48X mutation affects MOR28 OSN lateral coalescence during regeneration
(A–H) Projection of image stack obtained by light sheet microscopy (A, C, E, and G) and segmented to define projection patterns on the lateral surface (B, D, F, and H). (B, D, and H) Arrowhead indicate primary glomerular projection based on percent GFP expression relative to other projections (e.g., arrow in (B)). Asterisk in (B) indicates a projection that comprises less than 3% of the total GFP signal in the image stack. No primary projection could be easily determined in some samples (F).
(I) The number of lateral projections per half-bulb is increased in mutant and heterozygous animals.
(J) The percent of total GFP present in the main projection is significantly different between control and mutant and heterozygous mice.
(K) Histogram of distribution of GFP expression within each projection (greater than 3%) in the various genotypes. Scale bar: 100 μm. One-way ANOVA (I) F(3,21) = 6.16 (J) F(3,21) = 18.25, Dunnett’s test. ∗p < 0.05, ∗∗p < 0.01; ∗∗∗∗p < 0.0001. n = 7 (M cntrl), 8 (M mut), 5 (F mut), and 8 (F het).