Figure 4.
The Pcdh19E48X mutation affects MOR28 OSN medial coalescence during regeneration
(A–H) Projection of image stack obtained by light sheet microscopy (A, C, E, and G) and segmented to define projection patterns on the medial surface (B, D, F, and H). Arrowheads indicate primary glomerular projection based on percent GFP expression relative to other projections.
(I) The number of medial projections per half-bulb is increased in mutant and heterozygous animals; (J) The percent GFP present in the main medial projection is also different relative to control.
(K) Histogram of distribution of GFP expression within each projection (greater than 3%) in the various genotypes. Scale bar: 100 μm. One-way ANOVA (I) F(3,21) = 5.377 (J) F(3,21) = 5.382. Dunnett’s test. ∗p < 0.05, ∗∗p < 0.01. n=6 (M cntrl), 7 (M mut), 4 (F mut), and 8 (F het).