Male and female MOR28 OSNs can be distinguished based on gene expression
(A) Cluster analysis showing the most strongly differentially expressed genes between males and females. Note the variation in expression among different individual OSNs for any given gene.
(B) A subset of the same genes shown in (A) to indicate the difference in median expression between females and males.
(C) Single cell qRT-PCR shows that females tend to have higher expression of some genes. Each box represents a single cell; white boxes indicate higher expression. Pcdh9 (last two rows) is not predicted by the NanoString data to be statistically different between sexes, consistent with the qRT-PCR data.