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. 2023 Oct 29;26(11):108362. doi: 10.1016/j.isci.2023.108362

Figure 5.

Figure 5

Heterozygous deletion of Tmem106b does not rescue Grn −/− omic profile in 13 months old Grn −/− mice

(A and B) Volcano plots of lipidomic profiles comparing Grn−/− with WT cortex (A) or hippocampus (B). Red dots indicate significantly different lipids.

(C and D) BMP species levels were reduced similarly in Grn −/− and Tmem+/−, Grn−/− mice compared to Wt mice in cortex and hippocampus.

(E and F) Glucosylceramide and glucosylsphingosine levels were elevated in Grn−/− mice but to a lesser extent in Tmem +/−,Grn−/− mice in hippocampus by Mann Whitney U.

(G–N) Bulk RNAseq from isolated thalamic microglia. Select significant (FDR <0.05 & |Log2FC| > 1) differentially expressed genes from pairwise contrasts of Grn −/− vs. Wt and Tmem +/− Grn −/− vs. Grn −/−. N = 5–14 animals/genotype. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. All error bars represent S.E.M.