Figure 7.

Effect of thawing methods on post-thaw germination of cryopreserved pollen in Luffa species. (T1) thawing at room temperature of 25 °C (±2°C) for 20 min., (T2) thawing under running water at 20 °C (±5°C) for 10 min., (T3) thawing in water held at 35 °C for 5 min. with constant slow stirring, (T4) thawing under 10 °C for 5 min., and (T5) thawing under 10 °C for 2 min. followed by thawing at 35 °C for 3 min. Data presented in vertical bars represent mean ± SE. Significant differences (P ≤ 0.05) in pollen viability are presented by different alphabets analyzed by Duncan’s Multiple Range Test.