TABLE 1.
Oligonucleotide probes used in membrane hybridizations
| Probea | Target group | Optimum Td (°C) | Reference |
|---|---|---|---|
| S-*-Univ-1390-a-A-18 | Almost all organisms | 44 | 50 |
| S-D-Bact-0338-a-A-18 | Domain Bacteria | 56 | 6 |
| S-*-Myb-0736-b-A-22 | Mycolata | 51 | 17 |
| S-G-Gor-0596-a-A-22 | Genus Gordona | 54 | 17 |
| S-S-G.am-0205-a-A-19 | Gordona amarae | 53 | This study |
| S-*-G.am1-0439-a-A-19 | G. amarae group 1 strains | 66 | This study |
| S-*-G.am2-0439-a-A-19 | G. amarae group 2 strains | 61 | This study |
Probe names are standardized according to the Oligonucleotide Probe Database as follows: S or L for large- or small-subunit rRNA as the target; D for domain, O for order, F for family, G for genus, S for species, Ss for subspecies, and a wild-card symbol (∗) for universal probes, probes designed to target unidentified environmental isolates, or probes that do not circumscribe a coherent taxonomic group; letters designating the target group of the oligonucleotide probe; nucleotide position (E. coli numbering) in the target where the 3′ end of the probe binds; letter designating the version of the probe (a for version 1, b for version 2, etc.); S or A for sense or antisense direction; and number indicating the length (in nucleotides) of the probe (2).