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Evaluation of the immunogenicity of l-mGS2 and d-mGS2. Generation of an antidrug antibody (ADA) in mouse sera at days 0, 14, 28, 35, and 44 after injection of l-mGS2 and d-mGS2 was detected by ELISA (l-mGS2: n = 4; d-mGS2: n = 5). Absorbance of 3,3′,5,5′-tetramethylbenzidine (TMB) was measured at 450 nm. Statistical analysis was performed by two-way ANOVA followed by Sidak’s multiple comparisons test. *, p < 0.05, ***, p < 0.001.
