Table 1. SPR Analysis of Synthetic Monobodies Binding with EGFP.

Ligand	KD (nM)a
l-GS2biotin b	1.8 ± 1.3
l-mGS2biotin	9.8 ± 4.1
l-mGS2SS/biotin	3.7 ± 0.3
d-mGS2biotin	no binding

^aK_D values were determined from triplicate assays.

^bK_D value of recombinant GS2 was reported to be 3.4 ± 0.2 nM.²