Fig. 5.

MiR-146a-5p originated from hucMSC-exos could ameliorate the HTR8/SVneo cells injury induced by aPL through inhibiting TRAF6-mediated NF-κB signaling pathway. A-S HTR8/SVneo cells were transfected with miR-146a-5p mimic or inhibitor before the aPL-injury process. A and B The proliferation ability of HTR8/SVneo cells transfected with miR-146a-5p mimic (NC mimic) or miR-146a-5p inhibitor (NC inhibitor) was detected by EdU assay (n = 6). Scale bars, 20 μm. C and F The cell apoptotic rate was determined by flow cytometry assay with annexin-V/PI staining (n = 5). D, E and G Western blot analyzed the relative protein levels of inflammation-associated (IL-1β, IL-18) in HTR8/SVneo cells, quantified by signal intensity normalized to ACTIN (n = 3). H-J Transwell experiment detected the abilities of migration and invasion of HTR8/SVneo cells transfected with miR-146a-5p mimic (NC mimic) or inhibitor (NC inhibitor) (n = 5). Scale bars, 100 μm. K-N Western blot analyzed the relative protein levels of apoptosis-related (Cleaved-CASP3, BAX and BCL2) in HTR8/SVneo cells transfected with miR-146a-5p mimic (NC mimic) or miR-146a-5p inhibitor (NC inhibitor), quantified by signal intensity normalized to ACTIN (n = 3). O-S The mRNA expression levels of inflammation-associated (IL-1β, IL-18) and apoptosis-related (CASP3, BAX and BCL2) in the HTR8/SVneo cells transfected with miR-146a-5p mimic (NC mimic) or miR-146a-5p inhibitor (NC inhibitor) were analysed by qRT‒PCR (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001