Fig. 8.

HucMSC-exos alleviated the placental dysfunction in OAPS mice by delivering miR-146a-5p targeting the TRAF6/ NF-κB axis. A Representative images of FISH of miR-146a-5p in mice placenta sections. Scale bars, 20 µm. B and C Western blot analyzed the relative protein levels of TRAF6 and NF-κB p65 in mice palcenta, quantified by signal intensity normalized to ACTIN (n = 8). D and E Representative low- and high-magnification images of TRAF6 and NF-κB p65 IF staining in mice placenta (TRAF6, red; NF-κB, green; DAPI nuclear stain, blue), quantified by fluorescence intensity (n = 5). Scale bars, 500 μm and 20 μm. F-H Representative low- and high-magnification images of immunohistochemistry of TRAF6 and NF-κB p65 in mice placenta, quantified by average density (n = 5). Scale bars, 500 μm and 20 μm. I-N Western blot analyzed the relative protein levels of inflammation-associated (IL-1β, IL-18) and apoptosis-related (Cleaved-CASP3, BAX and BCL2) proteins in mice palcenta, quantified by signal intensity normalized to ACTIN (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001