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. 2023 Nov 13;13:207. doi: 10.1186/s13578-023-01166-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — GW4869 treatment affects the migration capability of the single-cell-derived clones. A, B Representative fluorescent images of the cell migration assays performed with the OPBG-DIPG002 derived clones (A) 1C5 (Venus) and 2B4 (m-Orange2), and the OPBG-GBM002 clones (B) 5E2 (Venus) and 1D3 (m-Orange2), in mono-culture and in co-culture (overlay of m-Orange2 and Venus) and in presence or absence of 10 μM and 20 μM of GW4869 or DMSO as vehicle control for 48 h. The graph bar shows the quantification of the cell migration areas after segmentation and analysis of the images using the Harmony software. Images were acquired with the Operetta CLS every 30 min over 48 h. At the 48 h time point, Scale bar = 200 μm. Data are mean ± SD, n = 2 with 6 different technical repeats. (****) p < 0.0001; (***) p < 0.001; (**) p < 0.01; (*) p < 0.05