Fig. 4.

Circ_16601 acts as a miR-5580-5p sponge to promote LUAD progression. A, B Relative expression of miR-5580-5p and miR-508-3p in A549 cells after circ_16601 overexpression or knockdown; C Relative expression of miR-5580-5p in NCI-H1299 cells after circ_16601 overexpression or knockdown; D RIP assay for circ_16601 was performed, and the coimmunoprecipitated RNA was subjected to RT‒qPCR to measure miR-5580-5p and miR-508-3p expression; E, F Colony formation, Transwell assays were performed to determine the proliferation and invasion ability of A549 cells after treatment. Scale bars: 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001