TABLE 1.
Signal transduction inhibitors and activators that affect sclerotial developmenta
| Compound | Concn (mM) | Sclerotial dry wt (mg)
|
No. of sclerotia
|
||
|---|---|---|---|---|---|
| Treatment | Controls | Treatment | Controls | ||
| Caffeine | 2.5 | 8.6 ± 6.5 | 26.3 ± 2.2 | 2.3 ± 1.7 | 5.0 ± 2.2 |
| 5.0 | 5.7 ± 5.2 | 27.2 ± 2.1 | 1.5 ± 1.6 | 5.8 ± 2.1 | |
| 10.0 | 0.8 ± 1.3 | 27.5 ± 1.4 | 0.3 ± 0.5 | 5.5 ± 2.4 | |
| IBMX | 2.5 | 16.3 ± 3.6 | 26.4 ± 1.7 | 3.3 ± 1.9 | 6.3 ± 2.7 |
| 5.0 | 14.0 ± 6.6 | 28.4 ± 1.8 | 3.8 ± 1.6 | 6.8 ± 1.2 | |
| 10.0 | 12.4 ± 6.4 | 26.6 ± 2.5 | 3.0 ± 1.9 | 5.3 ± 1.0 | |
| NaF | 0.5 | 5.5 ± 2.5 | 27.1 ± 2.7 | 4.5 ± 1.3 | 4.5 ± 1.7 |
| 1.0 | 5.1 ± 2.6 | 28.9 ± 1.9 | 4.0 ± 1.4 | 5.5 ± 1.0 | |
| 2.0 | 2.5 ± 2.0 | 28.4 ± 3.9 | 1.3 ± 1.0 | 5.0 ± 0.0 | |
a
S. sclerotiorum 1980 cultures were grown on PDA in 24-well tissue culture plates in the presence of different concentrations of caffeine, IBMX, and NaF. The average sclerotial dry weights and numbers of sclerotia produced per culture were determined by using data from four to six replicates.