TABLE 2.

Effect of caffeine on mycelial cAMP concentration^a

Time (h)	cAMP concn (fmol/mg)
	Treatment	Controls
72	180 ± 30	80 ± 30
96	800 ± 30	90 ± 30
120	790 ± 50	110 ± 10
144	1,000 ± 260	170 ± 40
168	550 ± 130	100 ± 40
192	460 ± 100	340 ± 10
216	440 ± 260	50 ± 0

^aS. sclerotiorum 1980 cultures were grown on cellophane discs overlaid on PDA (controls) or PDA containing 5 mM caffeine (treatment) in 24-well tissue culture plates. The data for each time point are the mean ± standard deviation for cAMP concentrations determined by a radioimmunoassay as described in Materials and Methods.