Study timeline. A: overall timeline of the study visits. Visits were separated by a washout period of at least 3 days. The last 3 days of the washout preceding each visit, diet, and food logs were provided, and participats were instructed to keep their diets and physical activity as consistent between visits as possible. B: sampling scheme for visits 1, 2, and 3 describing the timing of collection of height (Ht.), weight (Wt.), blood pressure (BP), and blood samples. C: sampling scheme for visit 4 describing the timing of collection of height (Ht.), weight (Wt.), blood pressure (BP), blood samples, and muscle biopsies. Figure was generated by BioRender. DEXA, dual X-ray absorptiometry; V̇o2max, maximal aerobic capacity.