Figure A1.

Heat map representation of 18S and 28S rRNAs recovered from metagenomes targeting material <0.2 μm during longitudinal study of asteroid wasting progression in control tissues, artificial scars, and wasting lesion margins. Red hues indicate greater numbers of library reads associated with taxonomy; black indicates no reads associated with taxonomy. There were no operational taxonomic units (OTUs) that were consistently present in wasting tissues across individuals but that were also absent from asymptomatic tissues on the same animal at the same time or in artificial scar tissues. C, asymptomatic tissue; A, artificial scar tissue; N, wasting lesion margin; T0, experiment initiation; Tf, time at death or for controls the end of observation period; Ti, time of appearance of lesion. Data derived from Hewson et al. (2020a).