Fig. 33. HomoFRET incorporating MPWs. (A) Schematic of dsDNA based MPW with a 3 TAMRA homoFRET relay. (B) Fluorescence spectra of homoFRET MPW and corresponding controls. (C) Cy5 fluorescence of spectra in panel B. Reproduced with permission from ref. 328 Copyright 2004 American Chemical Society. (D) Schematic of DX tile-based DNA MPW with a 6 Cy3.5 homoFRET relay. (E) Fluorescence spectra of MPW as the number of relay steps increased, R1–R6, signifies 1 Cy3.5 to 6 Cy3.5 respectively. (F) Eee values as a function of the length of the MPW. Reproduced with permission from ref. 147 Copyright, 2016, John Wiley & Sons.
