Fig. 34. HomoFRET on an origami breadboard. (A) Schematic of DNA origami breadboard containing triangular homoFRET relay. (B) Single particle FRET of the Cy3 relay dye to the final Cy5 accepter. (C) Fluorescence lifetime of Cy3 relay positions, it is notable that the lifetimes are similar but not indistinct as might be expected. (D) Fluorescence lifetime anisotropy of the same Cy3 configurations seen in C where localized influences on individual dyes were noted. Reproduced with permission from ref. 329 Copyright 2017 American Chemical Society.
