Fig. 38. Different homoFRET repeats as antennae in a DNA dendrimer. (A) Schematic of the DNA dendrimer with extendable homoFRET arms to increase LH. R1-R6 correlates with the number of Cy3 homoFRET dyes. (B) Fluorescence spectra of R1–R6. (C) Eee and Eea of varying LH structures as a function of the number of Cy3 homoFRET steps (i.e. R1–R6). As expected, the larger/longer structures have lower values. (D) Fluorescence spectral deconstruction of the R6 dendrimer showing the emission from the final Cy5.5 acceptor. (E) Predicted (line plot) and experimental (bar plot) AG of varying LH structures as a function of the number of Cy3 homoFRET steps (i.e., R1–R6). Here it is demonstrated how initial homoFRET additions increase the AG but subsequent additions are either neutral or impair LH. Reproduced with permission from ref. 151 Copyright, 2018, John Wiley & Sons.
