Fig. 63. Single-molecule FRET DNA barcoding. (A) Schematic presentation of the different stem, barcode, and probe DNAs (top) and the hybrid DNA complexes with six (Barcodes A, B, C, D, E, and F) FRET barcodes. (B) FRET efficiency distribution peaks (using one, two, three, or six barcode DNAs) collected from a single field of view. Solid red lines are Gaussian fits to each subpopulation. Dashed red lines present the position of each subpopulation. The peaks around 0.1 FRET efficiency correspond to the D only (no FRET) subpopulation. Reproduced with permission from ref. 438 Copyright 2021 American Chemical Society.