Fig. 69. RCA-FRET DNA-scaffold biosensor. (A) Biosensor combining one Tb D with two different dye As (Cy3.5 and Cy5.5). Hybridizing the DNA probes at different distances (bps) inside the target-specific RCA products provides four distinguishable signals (two distinct TG detection windows for each of the two emission colors). (B) Multiplexed quantification of four different DNA targets at different subpicomolar concentrations in 10 different samples. Reproduced with permission from ref. 436 Copyright 2018 American Chemical Society.
