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. 2023 Oct 24;52(22):7848–7948. doi: 10.1039/d0cs00936a

Overview of representative homoFRET MPWs.

DNA structure/estimated length # of homoFRET dyes HeteroFRET dyes Dye conjugation E ee Notes Ref.
25 bp ds triplex (∼8 nm) 2 TAMRA Eosin (D) Texas Red (A) Covalent ∼22% Pioneering study in this field 240
dsDNA/12 nm 3 TAMRA 6-Carboxyfluorescein (D) Cy5 (A) Covalent 20% Waveguide application 328
DX tile/31 nm 6 Cy3.5 A488 + Cy3 donors Covalent 2% Linear adjustable homoFRET module 147
Alexa647 + Cy5.5 acceptors
DX tile/24 nm 6 Cy3.5 Cy3 (D) and Alexa647 (A) Covalent 6% At 5 K in PVA film 146
Origami breadboard/16 nm 3 Cy3 A488 (D) and Cy5 (A) Covalent 3% Triangular homoFRET relay 329
Origami helix bundle/29 nm 10 Cy3.5 A488 (D) and A647 (A) Covalent 1% homoFRET antenna 149
Origami helix bundle/29 nm 6 Cy3.5 A488 (D) and A647 (A) Covalent 59% At 5 K in PVA film 149
dsDNA/16 nm YO-PRO-1 Pacific Blue (D) and Cy3 (A) Intercalation 20% 0.4 YO-PRO-1 dyes per bp 330
dsDNA/26 nm YO Pacific Blue (D) and Cy3 (A) Pyrrole-imidazole polyamide directed site-specific binding 14% Site-directed binding of YO dyes in the DNA scaffold 331