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miR-15a-5p, miR-34a-5p and miR-141-3p expression levels at plasma levels in PRAD (n=28) versus healthy controls (n=30) by qRT-PCR. For normalization of the miRNA expression data, U6 and RNU4 were analyzed based on the 2−ΔΔCT method (* p ≤ 0.05, **p≤ 0.01**** p ≤ 0.0001).