Figure 9.

Blocking Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) selectively depresses α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) excitatory postsynaptic currents (EPSCs). Inset shows the recording arrangement (black cell is control; the green cell lacks CaMKII). Time course of the effect of myr-CN27 (1 µM) on AMPAR EPSCs in wild-type (wt) cells (black circles) and simultaneously recorded CRISPR-CaMKIIα-transfected cells (green circles), normalized to wt baseline (from culture slices). Although myr-CN27 inhibited AMPAR EPSCs in wt cells, it had no effect on CRISPR-CaMKIIα-transfected cells (n = 6, P > 0.05, 2-tailed Wilcoxon signed rank test). Image from Ref. 361, with permission from eLife.