Figure 10.

Blocking Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) fully reverses established long-term potentiation (LTP). Diagram shows the two-pathway experimental setup. To record the response from two independent pathways, two bipolar stimulating electrodes were positioned to either side of the recorded cell with a distance of ∼100 μm. Stimuli were applied alternately every 20 s. The summary graph shows that myr-CN27 reduced the control pathway (black circles) 50% while completely reversing LTP (red circles) (difference between control and LTP pathway at 60 min; n = 11, P > 0.05, 2-tailed Wilcoxon signed rank test). Responses are means ± SE. Right: sample traces showing the effect of myr-CN27 on α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) excitatory postsynaptic currents (EPSCs) in control and LTP pathways. LTP is induced by 2-Hz stimulation for 90 s while holding the cell at 0 mV (361).