Figure 8.

Canonical pathways and upstream regulators between study 1 and study 2 in wild-type (WT) rats support changes in environmental stress between the 2 studies. The top canonical pathway in WT rats was the superpathway for cholesterol biosynthesis, although some genes had connection to the myelination signaling pathway. Notably, there is predicted activation of sterol regulatory element-binding factors 1 and 2 (SREBF1, SREBF2) in study 2 relative to study 1, which have sterol sensing functions. Red and green indicate up- and downregulated genes in study 2 relative to study 1, respectively. Orange and blue indicate Ingenuity Pathway Analysis predictions of activity, where orange indicates predicted activation while blue indicates predicted inhibition. Correspondingly, orange arrows suggest activation while blue arrows suggest inhibition; yellow arrows indicate that the prediction is inconsistent with the state of the downstream molecule.