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. 2023 Aug 2;325(3):E280–E290. doi: 10.1152/ajpendo.00051.2023

Figure 4.

Figure 4.

CCN2 induction in db/+ β cells does not induce β-cell proliferation but significantly decreases β-cell mass. β-cell proliferation after 2 wk of CCN2 induction from 4 to 6 wk of age (A) and 6 to 8 wk of age (B). β-cell mass after 2 wk of CCN2 induction from 4 to 6 wk of age (C) and 6 to 8 wk of age (D). n = 5–6 animals/group. E: qRT-PCR analysis of expression of cell death genes after 2 wk of DOX in db/+ control and db/+ animals with CCN2 induction. F: β-cell size in 8-wk-old db/+ animals after 2 wk of CCN2 induction. G: percent Pdx1+/insulin+ cells in 8-wk-old db/+ animals treated with DOX for 2 wk. Controls for 4- to 6-wk proliferation and mass studies: 4 db/+;RIP-rtTA, 2 db/+;TetO-CTGF. Control groups for 6- to 8-wk proliferation and mass studies: 2 db/+;RIP-rtTA, 3 db/+;TetO-CTGF. Control groups for Pdx1 study (6–8 wk): 2 db/+;RIP-rtTA, 1 TetO-CTGF. Control groups for β-cell size (6–8 wk): 2 db/+;RIP-rtTA, 1 TetO-CTGF. Data were analyzed using a Student’s t test. *P < 0.05. DOX, doxycycline.