Figure 6.

Influence of paracrine factors on nitric oxide (NO) production in human microvascular endothelial-cell (HMEC)-1 cells under different shear stress. Graphs showing the relative fluorescence of HMEC-1 cells under shear stress of 7 (A), 12 (B), 16 (C), or 20 (D) dyn/cm² over time for controls (Ctrl) or HMEC-1s treated with placental growth factor (PlGF), β-estradiol (β-est), progesterone (Prog), or the combination of all factors (Mix). Bar graphs showing the area under the curve (AUC) calculated for each condition are presented alongside. Data are from n = 3 or 4 biological replicates/group, run in technical duplicates. AUC was compared by one-way ANOVA + Dunnett’s post hoc test to each nontreated control group. Symbols and bar graphs show means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.