Table 1.
Paracrine factors and concentrations used for pressure myography and shear stress assays
Paracrine Factor | Manufacturer | Product Code | Concentration in Bath*/Media† | Reference(s) |
---|---|---|---|---|
Pressure myography experiments | ||||
β-Estradiol | Sigma-Aldrich | E4389 | 3 µM* | 35 |
Progesterone | Sigma-Aldrich | P7556 | 100 µM* | 36 |
Recombinant rat | ||||
PlGF protein | Abcam, UK | ab190989 | 0.1 nM* | 20 |
VEGF164 protein | Sigma-Aldrich | V3638 | 3 nM* | 32 |
In vitro shear stress assays | ||||
β-Estradiol | Sigma-Aldrich | E4389 | 36.7 nM† | 24, 37 |
Progesterone | Sigma-Aldrich | P7556 | 159 nM† | 24 |
Recombinant human | ||||
PlGF protein | PeproTech | 100-06 | 6.73 pM† | 25, 26 |
PlGF, placental growth factor; VEGF, vascular endothelial growth factor.