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. 2023 Aug 4;325(4):H790–H805. doi: 10.1152/ajpheart.00205.2023

Table 1.

Paracrine factors and concentrations used for pressure myography and shear stress assays

Paracrine Factor Manufacturer Product Code Concentration in Bath*/Media† Reference(s)
Pressure myography experiments
 β-Estradiol Sigma-Aldrich E4389 3 µM* 35
 Progesterone Sigma-Aldrich P7556 100 µM* 36
 Recombinant rat
  PlGF protein Abcam, UK ab190989 0.1 nM* 20
  VEGF164 protein Sigma-Aldrich V3638 3 nM* 32
In vitro shear stress assays
 β-Estradiol Sigma-Aldrich E4389 36.7 nM† 24, 37
 Progesterone Sigma-Aldrich P7556 159 nM† 24
 Recombinant human
  PlGF protein PeproTech 100-06 6.73 pM† 25, 26

PlGF, placental growth factor; VEGF, vascular endothelial growth factor.